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Liposome cocktail activator modulates hepatocytes and remodels the microenvironment to mitigate acute liver failure 
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Construction and characterization of C-activator. (A) Levels of mRNAs encoding growth factor HGF, EGF, IGF-1 and HSS were quantified using RT-qPCR ( n = 3; mean ± SD). (B) Levels of mRNAs encoding repair factor IL-4, IL-13, TGF-β and VEGF were quantified using RT-qPCR ( n = 3; mean ± SD). (C) Schematic diagram of the cocktail activator to realize hepatocyte rescue and inflammation modulation by DMI-loaded liposomes modified with DG. (D) Particle size distribution and TEM image of C-activator. Scar bar is 200 nm. (E-F) Mean fluorescence intensity and flow cytometry graphs of cellular uptake of RhB-Lip (32 µg/ml) by <t>LO2</t> cells after incubation for 1, 2, 4, 6, 8 and 10 h ( n = 3; mean ± SD). * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001 compared with APAP group by one-way ANOVA test or two-way ANOVA test.
Liposome cocktail activator modulates hepatocytes and remodels the microenvironment to mitigate acute liver failure 
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In vitro cell viability, antioxidant activities, and anti-inflammatory properties of C-activator in APAP-injured <t>LO2</t> cells. (A) Cell viability of LO2 cells exposure to C-activator in the presence of APAP (10 mM) for 24 h ( n = 6; mean ± SD). (B) Confirmation of generation and inhibition of ROS in LO2 cells undergoing C-activator treatment, scale bars are 400 µm. (C-D) Intracellular generation of ROS in LO2 cells detected by flow cytometry using DCFH-DA probe ( n = 3; mean ± SD). (E-G) Total RNA extracted from LO2 cells was measured by RT-qPCR and the expression levels of proinflammatory cytokines IL-6, IL-1β and TNF-α were normalized to the mRNA level of β-actin ( n = 3; mean ± SD). * P < 0.05, ** P < 0.01, **** P < 0.0001 compared with APAP group by one-way ANOVA test.
Liposome cocktail activator modulates hepatocytes and remodels the microenvironment to mitigate acute liver failure 
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C-activator conditioned medium enhanced cell viability after APAP-induced hepatocyte damage. (A) A schematic illustrating the experimental protocol for conditioned medium (CM) administration. (B-C) Cell viability of <t>LO2</t> cells treated with different CM was evaluated by crystal violet staining and quantified by measuring the absorbance at 570 nm ( n = 3; mean ± SD). (D) Succinyl dehydrogenase (SDH) viability of LO2 cells treated with CM was measured using MTT assay ( n = 6; mean ± SD). ** P < 0.01, **** P < 0.0001 compared with APAP CM group by one-way ANOVA test and ## P < 0.01, #### P < 0.0001 compared with C-activator CM group by one-way ANOVA test.
Liposome cocktail activator modulates hepatocytes and remodels the microenvironment to mitigate acute liver failure Asian Journal of Pharmaceutical Sciences, 2022 Oct 31
"Construction and characterization of C-activator. (A) Levels of mRNAs encoding growth factor HGF, EGF, IGF-1 and HSS were quantified using RT-qPCR ( n = 3; mean ± SD). (B) Levels of mRNAs encoding repair factor IL-4, IL-13, TGF-β and VEGF were quantified using RT-qPCR ( n = 3; mean ± SD). (C) Schematic diagram of the cocktail activator to realize hepatocyte rescue and inflammation modulation by DMI-loaded liposomes modified with DG. (D) Particle size distribution and TEM image of C-activator. Scar bar is 200 nm. (E-F) Mean fluorescence intensity and flow cytometry graphs of cellular uptake of RhB-Lip (32 µg/ml) by <t>LO2</t> cells after incubation for 1, 2, 4, 6, 8 and 10 h ( n = 3; mean ± SD). * P < 0.05, ** P <0.01, *** P < 0.001, **** P < 0.0001 compared with APAP group by one-way ANOVA test or two-way ANOVA test. "
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